Transgenic tomato expressing an oxalate decarboxylase gene from Flammulina sp. shows increased survival to Moniliophthora perniciosa

• oxdc gene from flammulina velutipes was used to generate transgenic tomato plants. Transgenic T 1 lines showed a significant delay in symptom development. higher survival percentage witches’ broom disease. The P16 line decreased production of H 2 O the presence oxalic acid. Witches’ disease progression reduced by gene. which affects cacao tree ( Theobroma L.) is caused hemibiotrophic fungus Moniliophthora perniciosa . Studies cacao- M. interaction that pathogenicity associated acid (OA). In some organisms, OA can be degraded into formate and CO oxalate decarboxylase (OXDC) enzyme. Here, we Flammulina plants as potential strategy control infection. Three expressing were obtained two them (P4 P16) presented development when inoculated with comparison non-transformed wild-type (WT) Moreover, test WT, evidenced 3,3′-diaminobenzidine test. These results indicate able break down leading normal plant few symptoms even infected Phenotypic histological analysis differences between WT amount hyphae lower thinner thickness This study evidences reduction survival.